Write a summary information about:
1.Enzymes in general ( 1 paragraph)
2.Enzyme regulation mentioning pH, temperature and highlighting the enzyme inhibitors (1 paragraph)
3.Enzyme kinetics-Write the most important equations (Michaelis-Menten and Lineweaver-Burk) and the derived graphs; describe their key parameters ( Definitions of Km and Vmax) (2 paragraphs).
4.The enzyme ALP and the clinical importance of this enzyme(1 paragraph)
5.Aim of the experiment: What is the problem you are trying to solve? (enzyme kinetics and regulation (pH and temperature) for ALP enzyme).
Your introduction should be supported by the use of journal references (cited using Harvard referencing style-see library).
1) Title, your name and the module
The title should be explanatory, concrete and referred to the problem studied
Correct: Determination of the ALP activity: Enzyme kinetic analysis and regulation.
Incorrect: Enzyme experiment
Incorrect: Analysis of ALP
Incorrect: Experiment Week 3-5
Write a summary information about:
1.Enzymes in general ( 1 paragraph)
2.Enzyme regulation mentioning pH, temperature and highlighting the enzyme inhibitors (1 paragraph)
3.Enzyme kinetics-Write the most important equations (Michaelis-Menten and Lineweaver-Burk) and the derived graphs; describe their key parameters ( Definitions of Km and Vmax) (2 paragraphs).
4.The enzyme ALP and the clinical importance of this enzyme(1 paragraph)
5.Aim of the experiment: What is the problem you are trying to solve? (enzyme kinetics and regulation (pH and temperature) for ALP enzyme).
Your introduction should be supported by the use of journal references (cited using Harvard referencing style-see library).
Provide a detailed account of your findings. Include fully-labelled and titled results, and graphs.
- First, prepare all the necessary graphs in your excel sheet and calculate the kinetic parameters (Km and Vmax (with or without inhibitor).
- Explain briefly the experimental data and your observations.
- Write also observations while being in the lab (e.g. change in colour in your reaction tubes etc).
- Insert the 4 Graphs from the excel and explain them.
- Prepare 1 table with the values of Km and Vmax (with and without inhibitor).Summarize and discuss your results, the cause of the possible trouble shootings (if any) and relate them to your findings with the bibliography.
- Explain the enzyme kinetic analysis for ALP and compare your results with information about ALP in the literature.
- Explain what type of inhibitor is the Na2HPO4 and discuss your results comparing them to the literature. What other experiments should you do to further confirm your conclusion?
- Discuss the effect of temperature on ALP activity by citing other references mentioning the regulation by this factor.Briefly summarise the main findings and highlight the most important results and their importance!
