How does the order of elution from a column relate to Rf values in TLC? Which of the four compounds shown in Figure 1 has the highest Rf value in TLC? Why?

Experiment 13

Questions for Data Analysis and Results

Based on the structures shown in Figure 1, rank the four compounds in order of polarity (least polar to most polar). Then state the order of elution from the column (first to elute to last to elute).

Recall that a green band was left on the column. Why did that band remain on the column? What could you do in the procedure to elute that band?

How does the order of elution from a column relate to Rf values in TLC? (For example, does the first compound to elute from the column have the highest or lowest Rf value in TLC?)

Which of the four compounds shown in Figure 1 has the highest Rf value in TLC? Why? Which has the lowest Rf value in TLC? Why?

Were there likely additional compounds (besides one of the four listed in Figure 1) remaining on the column? How do you know?